Thin layer chromatography (TLC) is a simple, fast and highly versatile separation tool for both qualitative and quantitative analyses. The technique can be used to resolve virtually all classes of substances, including pesticides, steroids, alkaloids, lipids, nucleotides, glycosides, carbohydrates, fatty acids and many others. Apart from the manual method of classical TLC, the technique can also be automated as in instrumental high performance thin layer chromatography (HPTLC). Furthermore, it can be easily extended to preparative scale for PLC.
Thin Layer Chromatography